BAP31 is involved in T cell activation through TCR signal pathways
Citations Over TimeTop 14% of 2017 papers
Abstract
BAP31 is a ubiquitously expressed endoplasmic reticulum (ER) membrane protein. The functions of BAP31 in the immune system have not been investigated due to the lack of animal models. Therefore we created a BAP31 conditional knockdown mouse by performing a knockdown of BAP31 in the thymus. In doing so, we demonstrate that the maturation of T cells is normal but the number of T cells is less in the thymus of the knockout mouse. In addition, the spleen and lymph nodes of peripheral immune organs contained a lesser proportion of the mature T cells in the thymus specific BAP31 knockout mice. The BAP31 knockout T cells decreased the proliferation activated by TCR signal pathways. Further studies clarified that BAP31 affects the phosphorylation levels of both Zap70/Lck/Lat of the upstream members and Akt/GSK/Jnk/Erk of the downstream members of TCR signal pathways. Furthermore, BAP31 can regulate the expression of some markers such as CD3/TCRα/TCRβ and some cytokines like IL-2/IFN-γ/IL-6/TNF-α which are important for T cell activation. Taken together, these results demonstrate that BAP31 may play an important role in T cell activation by regulating TCR signaling.
Related Papers
- → Some Hints Concerning the Shape of T‐Cell Receptor Structures(2002)12 cited
- → Identification of αβ and γδ T Cell Receptor‐Positive Cells(1988)6 cited
- → A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice(2023)2 cited
- → Identifying transcriptional changes in an oligodendrocyte‐specific conditional knockout mouse (934.6)(2014)
- Impacto fenotípico y funcional de quimeras de cd3gamma y cd3 delta en el complejo tcr/cd3 humano deficiente de cd3gamma(2009)