Dissolution of Candida antarctica lipase B in ionic liquids: effects on structure and activity
Green Chemistry2004Vol. 6(9), pp. 483–483
Citations Over TimeTop 10% of 2004 papers
Rute Madeira Lau, Menno J. Sorgedrager, Giacomo Carrea, Fred van Rantwijk, Francesco Secundo, Roger A. Sheldon
Abstract
The effects of ionic liquid media on the activity of Candida antarctica lipase B in a simple transesterification reaction were studied. In ionic liquids containing alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF4]. Only [Et3MeN][MeSO4] was an exception, as dissolved CaLB maintained its activity in this solvent. By means of FT-IR spectroscopy, denaturation of CaLB was observed upon dissolution in ionic liquids in which the activity was low, whereas the conformation of enzyme dissolved in [Et3MeN][MeSO4] closely resembled the native one.
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