Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability

Citations Over TimeTop 10% of 2009 papers

Abstract

This paper describes a microfluidic device for the microencapsulation of cells in alginate beads to enhance cell viability. The alginate droplet including cells was gelified with calcified oleic acid, using two-phase microfluidics. The on-chip gelation had generated monodisperse spherical alginate beads, which could not be readily obtained via conventional external gelation in a calcium chloride bath. However, the prolonged exposure of encapsulated cells to the toxic oil phase caused serious damage to the cells. Therefore, we proposed the formulation of a rapid oil-exchange chip which transforms the toxic oleic acid to harmless mineral oil. The flushing out of oleic acid after the gelation of alginate beads effected a dramatic increase in the viability of P19 embryonic carcinoma cells, up to 90%. The experimental results demonstrated that the cell viability was proportional to the flow rate of squeezing mineral oil.

Related Papers

• → Dispersity in polymer science (IUPAC Recommendations 2009)(2009)306 cited
• → Controlling primary chain dispersity in network polymers: elucidating the effect of dispersity on degradation(2023)20 cited
• → Dispersity in polymer science (IUPAC Recommendation 2009)(2009)33 cited
• → Distribution of near neighbours in randomly packed hard spheres(1980)8 cited
• → Dispersity in Polymer Science(2016)