Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells
Citations Over TimeTop 1% of 2009 papers
Abstract
Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.
Related Papers
- → Low-Temperature Grinding Technique for Infrared Analysis of Propellants and Binders in Potassium Bromide Disks(1968)2 cited
- Effects of Quality of Potassium Bromide on IR Spectrum Testing(2010)
- Purification of Potassium Bromide Used for Infrared Spectral Analysis(2011)
- → Studies on Photoelectric Effect and its Medical Applications. : Seventh Report. The Excretion of Potassium Bromide of Healthy Persons.(1938)
- → Application of Fourier transform infrared-photoacoustic spectroscopy for the characterisation of organic wastes and determination of their usefulness for bioenergy and as soil amendments(2016)