Merely two mutations switch a DNA-hydrolyzing deoxyribozyme from heterobimetallic (Zn2+/Mn2+) to monometallic (Zn2+-only) behavior

Citations Over TimeTop 18% of 2010 papers

Abstract

A deoxyribozyme that hydrolyzes DNA phosphodiester linkages with a requirement for both Zn(2+) and Mn(2+) is switched by only two nucleotide mutations to require Zn(2+) alone, demonstrating that DNA-catalyzed DNA hydrolysis can be achieved using only one metal ion cofactor.

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