An optimized isotopic labelling strategy of isoleucine-γ2methyl groups for solution NMR studies of high molecular weight proteins
Chemical Communications2011Vol. 48(10), pp. 1434–1436
Citations Over TimeTop 19% of 2011 papers
Isabel Ayala, Olivier Hamelin, Carlos Amero, Ombeline Pessey, Michael J. Plevin, Pierre Gans, Jérôme Boisbouvier
Abstract
An efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-γ(2) groups in proteins. The (2)H, (13)C-pattern of the biosynthetic precursor has been designed to optimize magnetization transfer, in large proteins, between these important structural probes and their corresponding backbone nuclei.
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