Rational design of boron dipyrromethene (BODIPY)-based photobleaching-resistant fluorophores applicable to a protein dynamics study
Chemical Communications2011Vol. 47(36), pp. 10055–10055
Citations Over TimeTop 14% of 2011 papers
Toru Komatsu, Daihi Oushiki, Aoi Takeda, M. Miyamura, Tasuku Ueno, Takuya Terai, Kenjiro Hanaoka, Yasuteru Urano, Tomoko Mineno, Tetsuo Nagano
Abstract
We studied the photobleaching of a library of boron dipyrromethene (BODIPY) derivatives with a range of electron densities, and found that the photobleaching rate is influenced by the electron-withdrawing capacity of the substituents. Electron-deficient BODIPYs generated less singlet oxygen, were less reactive to singlet oxygen, and were highly resistant to photobleaching. We confirmed the utility of one of these fluorophores, 2,6-diCO(2)R-BDP, for visualizing EGF receptor dynamics in cells expressing an SNAP-tagged EGF receptor.
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