Rational substrate and enzyme engineering of transketolase for aromatics
Organic & Biomolecular Chemistry2012Vol. 10(45), pp. 9021–9021
Citations Over TimeTop 13% of 2012 papers
Panwajee Payongsri, David Steadman, John Strafford, Andrew MacMurray, Helen C. Hailes, Paul A. Dalby
Abstract
The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.
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