Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe
Chemical Communications2013Vol. 49(50), pp. 5630–5630
Citations Over TimeTop 10% of 2013 papers
Ka‐Ho Leung, Hong‐Zhang He, Victor Pui‐Yan, Hai‐Jing Zhong, Daniel Shiu‐Hin Chan, Jun Zhou, Jean‐Louis Mergny, Chung‐Hang Leung, Dik‐Lung Ma
Abstract
We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.
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