Highly sensitive fluorescent protein FRET detection using optofluidic lasers
Lab on a Chip2013Vol. 13(14), pp. 2679–2679
Citations Over TimeTop 1% of 2013 papers
Abstract
We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.
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