High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues
Chemical Communications2018Vol. 54(96), pp. 13531–13534
Citations Over TimeTop 10% of 2018 papers
Min Jung Chang, Kyutae Kim, Kyun Seob Park, Ji Su Kang, Chang Su Lim, Hwan Myung Kim, Chulhun Kang, Min Hee Lee
Abstract
We developed a fluorescent pH probe (1) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide-piperazine-rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image the pH perturbations of mitochondria in living cells and tissues.
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