Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation
Biochemical Journal1992Vol. 286(2), pp. 331–334
Citations Over TimeTop 1% of 1992 papers
Abstract
Apoptosis, a major form of cell death, is characterized by chromatin condensation, a reduction in cell volume and endonuclease cleavage of DNA into oligonucleosomal length fragments. The detection of these fragments by gel electrophoresis, as a DNA ladder, is currently used as the major biochemical index of apoptosis. Here we report that key morphological changes of apoptosis can be dissociated experimentally from the DNA fragmentation produced by endonuclease activity. Internucleosomal cleavage of DNA is thus likely to be a later event in the apoptotic process.
Related Papers
- → An overview of apoptosis assays detecting DNA fragmentation(2018)348 cited
- → TUNEL Assay: An Overview of Techniques(2003)60 cited
- → Detection of apoptotic cells based on in situ hybridization chain reaction using specific hairpins(2022)4 cited
- → New aspects of the mechanism of DNA fragmentation in apoptosis(1997)139 cited
- → Detection of DNA Fragmentation in Apoptotic Cells by TUNEL(2016)95 cited