Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs

Citations Over TimeTop 10% of 2001 papers

Abstract

The absence of expression of the granule-bound starch synthase I (GBSSI) allele from chromosome 4A of wheat is associated with improved starch quality for making Udon noodles. Several PCR-based methods for the analysis of GBSS alleles have been developed for application in wheat. A widely applied approach has involved a simple PCR followed by electrophoretic separation of DNA products on agarose gels. The PCR amplifies one band from each of the loci on chromosomes 4A ( Wx-B1 ), 7A ( Wx-A1 ), and 7D ( Wx-D1 ), and the band from the Wx-B1 locus is diagnostic for the occurrence of the null Wx-B1 allele that is associated with improved starch quality. The reliable detection of the null Wx-B1 allele has been important in identifying wheat breeding lines. Allele-specific PCR has also been used to successfully detect the occurrence of the null Wx-B1 allele. In the present paper the various protocols were evaluated by testing a segregating double haploid population from a cross between Cranbrook and Halberd and the tests gave good agreement in different laboratories. The application of the DNAbased tests applied in wheat breeding programs provides one of the first examples of a molecular marker selection for a grain quality trait being successfully applied in an Australian wheat breeding program.

Related Papers

• → Haploid male fertility is restored by parallel spindle genes in Arabidopsis thaliana(2023)15 cited
• → In vitro production of haploid and doubled haploid plants from pollinated ovaries of maize (Zea mays)(2005)52 cited
• → Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision In Vitro(2018)8 cited
• → Genetic analysis of anther-derived plants of rice(1982)35 cited
• → Genetic Variation among Doubled Haploid Lines of Burley Tobacco Varieties(1974)30 cited