Cell-type-specific signatures of microRNAs on target mRNA expression
Citations Over TimeTop 1% of 2006 papers
Abstract
Although it is known that the human genome contains hundreds of microRNA (miRNA) genes and that each miRNA can regulate a large number of mRNA targets, the overall effect of miRNAs on mRNA tissue profiles has not been systematically elucidated. Here, we show that predicted human mRNA targets of several highly tissue-specific miRNAs are typically expressed in the same tissue as the miRNA but at significantly lower levels than in tissues where the miRNA is not present. Conversely, highly expressed genes are often enriched in mRNAs that do not have the recognition motifs for the miRNAs expressed in these tissues. Together, our data support the hypothesis that miRNA expression broadly contributes to tissue specificity of mRNA expression in many human tissues. Based on these insights, we apply a computational tool to directly correlate 3' UTR motifs with changes in mRNA levels upon miRNA overexpression or knockdown. We show that this tool can identify functionally important 3' UTR motifs without cross-species comparison.
Related Papers
- → Abundant conserved microRNA target sites in the 5′-untranslated region and coding sequence(2009)87 cited
- → The Effects of ABCB1 3′-Untranslated Region Variants on mRNA Stability(2007)17 cited
- Constructing series of clones with different functional regions deletion of PEPV 5′Untranslated region and sequence analysis(2007)
- Sequences near both termini of the C/EBPβ mRNA 3＇ untranslated region are important for its tumor suppression activity(2009)
- 食管癌细胞SHEEC中NGAL基因5’—UTR和3’—UTR的克隆与鉴定(2003)