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Use of Real‐Time PCR on Blood Samples for Diagnosis of Invasive Aspergillosis

Clinical Infectious Diseases2001Vol. 33(9), pp. 1504–1512

Citations Over TimeTop 10% of 2001 papers

Masahiro Kami, Takafumi Fukui, Seishi Ogawa, Yukimasa Kazuyama, Utako Machida, Yuji Tanaka, Yoshinobu Kanda, Takeshi Kashima, Yasuaki Yamazaki, Tamae Hamaki, Shinichiro Mori, Hideki Akiyama, Yoshitomo Mutou, Hisashi Sakamaki, Kazuoki Osumi, Satoshi Kimura, Hisamaru Hirai

Abstract

We developed a new quantitative system for diagnosis of invasive pulmonary aspergillosis (IPA) using real-time automated polymerase chain reaction (PCR). Intra-assay and interassay precision rates for in vitro examination were 2.53% and 2.20%, respectively, and the linearity of this assay was obtained when there were >20 copies/well. We examined 323 samples taken from 122 patients with hematological malignancies, including 33 patients with IPA and 89 control patients. Blood samples were subjected to PCR antigen detection methods, using enzyme-linked immunosorbent assay (ELISA) and determination of plasma (1-->3)-beta-D-glucan (BDG) concentration. The sensitivities of PCR, ELISA, and BDG measurement for diagnosis of IPA were 79%, 58%, and 67%, respectively; the specificities were 92%, 97%, and 84%. Positive findings on PCR preceded those of computed tomography by -0.3+/-6.6 days, those of BDG measurement by 6.5+/-4.9 days, and those of ELISA by 2.8+/-4.1 days. Real-time PCR was sensitive for IPA diagnosis, and quantitation was accurate.

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