DNA Immunization for Influenza Virus: Studies Using Hemagglutinin‐ and Nucleoprotein‐Expressing DNAs
The Journal of Infectious Diseases1997Vol. 176(s1), pp. S50–S55
Citations Over TimeTop 10% of 1997 papers
Harriet L. Robinson, Christine Boyle, David Feltquate, Merribeth J. Morin, Joseph C. Santoro, Robert G. Webster
Abstract
DNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/8/34 (H1N1), challenge. The HA DNA, but not the NP DNA, protected mice against the lethal viral challenge. For the HA DNA, single gene gun inoculations of 0.04 microg and boosted inoculations of 0.004 microg of DNA raised complete protection. For the NP DNA, boosted gene gun immunizations of 0.4 microg of DNA and boosted intradermal or intramuscular injections of 50 microg of DNA failed to protect. The protection elicited by the HA DNA vaccine correlated with the titers of neutralizing antibody.
Related Papers
- → Strategies for inducing protection against avian influenza A virus subtypes with DNA vaccines(2000)123 cited
- → Detection of Nonhemagglutinating Influenza A(H3) Viruses by Enzyme-Linked Immunosorbent Assay in Quantitative Influenza Virus Culture(2014)36 cited
- → Development of an Immunochromatographic Assay Specifically Detecting Pandemic H1N1 (2009) Influenza Virus(2010)31 cited
- → DNA vaccines: A novel approach to immunization(1995)89 cited
- → Live attenuated influenza virus vaccines strains with temperature-sensitive defects in P3 protein and nucleoprotein(1977)40 cited