Neural Alterations and Hyperactivity of the Hypothalamic–Pituitary–Thyroid Axis in Oatp1c1 Deficiency

Citations Over TimeTop 11% of 2019 papers

Abstract

Background: The thyroid hormones (THs) triiodothyronine (T3) and thyroxine (T4) are crucial regulators of brain development and function. Cell-specific transporter proteins facilitate TH uptake and efflux across the cell membrane, and insufficient TH transport causes hypothyroidism and mental retardation. Mutations in the TH transporters monocarboxylate transporter 8 (MCT8, SLC16A2 ) and the organic anion-transporting polypeptide 1C1 (OATP1C1, SLCO1C1 ) are associated with the psychomotor retardation Allan-Herndon-Dudley syndrome and juvenile neurodegeneration, respectively. Methods: To understand the mechanisms and test potential treatments for the recently discovered OATP1C1 deficiency, we established an oatp1c1 mutant ( oatp1c1 −/− ) zebrafish. Results: oatp1c1 is expressed in endothelial cells, neurons, and astrocytes in zebrafish. The activity of the hypothalamic–pituitary–thyroid axis and behavioral locomotor activity increased in oatp1c1 −/− larvae. Neuropathological analysis revealed structural alteration in radial glial cells and shorter neuronal axons in oatp1c1 −/− larvae and adults. Notably, oatp1c1 −/− and oatp1c1 −/− X mct8 −/− adults exhibit an enlarged thyroid gland (goiter). Pharmacological assays showed that TH analogs, but not THs, can reduce the size and improve the color of the thyroid gland in adult mutant zebrafish. Conclusion: These results establish a vertebrate model for OATP1C1 deficiency that demonstrates endocrinological, neurological, and behavioral alterations mimicking findings observed in an OATP1C1-deficient patient. Further, the curative effect of TH analogs in the oatp1c1 −/− zebrafish model may provide a lead toward a treatment modality in human patients.

Related Papers

• → Cold exposure down-regulates zebrafish pigmentation(2011)16 cited
• Susquehanna Chorale Spring Concert "Roots and Wings"(2017)
• → Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο(2016)
• → Διερεύνηση μηχανισμών ανάπτυξης καρδιαγγειακών νόσων χρησιμοποιώντας το πειραματόζωο Danio rerio (zebrafish)(2020)
• → Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο(2020)