Draft Genome of the Pearl Oyster Pinctada fucata: A Platform for Understanding Bivalve Biology
Citations Over TimeTop 1% of 2012 papers
Abstract
The study of the pearl oyster Pinctada fucata is key to increasing our understanding of the molecular mechanisms involved in pearl biosynthesis and biology of bivalve molluscs. We sequenced ~1150-Mb genome at ~40-fold coverage using the Roche 454 GS-FLX and Illumina GAIIx sequencers. The sequences were assembled into contigs with N50 = 1.6 kb (total contig assembly reached to 1024 Mb) and scaffolds with N50 = 14.5 kb. The pearl oyster genome is AT-rich, with a GC content of 34%. DNA transposons, retrotransposons, and tandem repeat elements occupied 0.4, 1.5, and 7.9% of the genome, respectively (a total of 9.8%). Version 1.0 of the P. fucata draft genome contains 23 257 complete gene models, 70% of which are supported by the corresponding expressed sequence tags. The genes include those reported to have an association with bio-mineralization. Genes encoding transcription factors and signal transduction molecules are present in numbers comparable with genomes of other metazoans. Genome-wide molecular phylogeny suggests that the lophotrochozoan represents a distinct clade from ecdysozoans. Our draft genome of the pearl oyster thus provides a platform for the identification of selection markers and genes for calcification, knowledge of which will be important in the pearl industry.
Related Papers
- → Quantitative expression of shell matrix protein genes and their correlations with shell traits in the pearl oyster Pinctada fucata(2011)31 cited
- → Expression profiles of nine biomineralization genes and their relationship with pearl nacre thickness in the pearl oyster,Pinctada fucata martensiiDunker(2014)8 cited
- → Seasonal and positional variations in the rate of nacre coating in Indian pearl oyster Pinctada fucata (Gould, 1850)(2018)2 cited
- → PU14, a Novel Matrix Protein, Participates in Pearl Oyster, Pinctada Fucata, Shell Formation(2021)2 cited
- → Pearl Production by Implantation of Outer Epithelial Cells Isolated from the Mantle of Pinctada fucata and the Effects of Blending of Epithelial Cells with Different Genetic Backgrounds on Pearl Quality(2018)1 cited