Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2
bioRxiv (Cold Spring Harbor Laboratory)2017
Citations Over Time
Pavel Dvořák, Pavel Dvořák, David Bednář, Pavel Vaňáček, Lukáš Bálek, Lívia Eiselleová, Veronika Štěpánková, Eva Šebestová, Michaela Kunova Bosakova, Žaneta Konečná, Stanislav Mazurenko, Antonín Kunka, Daniel Horák, Radka Chaloupková, Jan Brezovský, Pavel Krejčı́, Zbyněk Prokop, Petr Dvorak, Petr Dvorak, Jiřı́ Damborský
Abstract
Abstract Fibroblast growth factors (FGFs) play numerous regulatory functions in complex organisms, and their corresponding therapeutic potential is of growing interest to academics and industrial researchers alike. However, applications of these proteins are limited due to their low stability in vivo and in vitro . Here we tackle this problem using a generalizable computer-assisted protein engineering strategy to create a unique modified FGF2 with nine mutations displaying unprecedented stability and uncompromised biological function.
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