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Glutathione levels influence chronological life span of Saccharomyces cerevisiae in a glucose-dependent manner

bioRxiv (Cold Spring Harbor Laboratory)2017

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Mayra Fabiola Tello‐Padilla, Alejandra Yudid Perez‐Gonzalez, Melina Canizal‐García, Juan Carlos González‐Hernández, Christian Cortés‐Rojo, Ivanna Karina Olivares‐Marin, Luis Alberto Madrigal‐Pérez

Abstract

Abstract Diet plays a key role in determining the longevity of the organisms since it has been demonstrated that glucose restriction increases lifespan whereas a high-glucose diet decreases it. However, the molecular basis of how diet leads to the aging process is currently unknown. We propose that the quantity of glucose that fuels respiration influences ROS generation and glutathione levels, and both chemical species impact in the aging process. Herein, we provide evidence that mutation of the gene GSH1 diminishes glutathione levels. Moreover, glutathione levels were higher with 0.5% than in 10% glucose in the gsh1Δ and WT strains. Interestingly, the chronological life span (CLS) was lowered in the gsh1Δ strain cultured with 10% glucose but not under dietary restriction. The gsh1Δ strain also showed an inhibition of the mitochondrial respiration in 0.5 and 10% of glucose but only increased the H 2 O 2 levels under dietary restriction. These results correlate well with the GSH/GSSG ratio, which showed a decrease in gsh1Δ strain cultured with 0.5% glucose. Altogether these data indicate that glutathione has a major role in the function of electron transport chain (ETC) and is essential to maintain life span of Saccharomyces cerevisiae in 10% glucose.

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Abstract

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