Panoptic vDISCO imaging reveals neuronal connectivity, remote trauma effects and meningeal vessels in intact transparent mice
Citations Over Time
Abstract
Analysis of entire transparent rodent bodies could provide holistic information on biological systems in health and disease. However, it has been challenging to reliably image and quantify signal from endogenously expressed fluorescent proteins in large cleared mouse bodies due to the low signal contrast. Here, we devised a pressure driven, nanobody based whole-body immunolabeling technology to enhance the signal of fluorescent proteins by up to two orders of magnitude. This allowed us to image subcellular details in transparent mouse bodies through bones and highly autofluorescent tissues, and perform quantifications. We visualized for the first-time whole-body neuronal connectivity of an entire adult mouse and discovered that brain trauma induces degeneration of peripheral axons. We also imaged meningeal lymphatic vessels and immune cells through the intact skull and vertebra in naive animals and trauma models. Thus, our new approach can provide an unbiased holistic view of biological events affecting the nervous system and the rest of the body.
Related Papers
- → 2D and 3D immunogold localization on (epoxy) ultrathin sections with and without osmium tetroxide(2020)13 cited
- → Doublecortin immunolabeling in canine gliomas with distinct degrees of tumor infiltration(2022)3 cited
- → Postembedding Immunolabeling of Thin Sections of Drosophila Tissues for Transmission Electron Microscopy(2012)2 cited
- → Freeze-substitution in Epon: An attempt to combine immunolabeling and improvement of structural preservation(2008)
- → Specimen Preparation for Immunocytochemistry(2001)