Widespread cytoplasmic polyadenylation programs asymmetry in the germline and early embryo

Citations Over Time

Abstract

Abstract BACKGROUND The program of embryonic development is launched by selective activation of a silent maternal transcriptome. In Caenorhabditis elegans , nuclei of the adult germline are responsible for the synthesis of at least two distinct mRNA populations; those required for housekeeping functions, and those that program the oocyte-to-embryo transition. We mapped this separation by changes to the length-distribution of poly(A)-tails that depend on GLD-2 mediated cytoplasmic polyadenylation and its regulators genome-wide. RESULTS More than 1000 targets of cytoplasmic polyadenylation were identified by differential polyadenylation. Amongst mRNA with the greatest dependence on GLD-2 were those encoding RNA binding proteins with known roles in spatiotemporal patterning such as mex-5 and pos-1 . In General, the 3’ UTR of GLD-2 targets were longer, contained cytosine-patches, and were enriched for non-standard polyadenylation-motifs. To identify the deadenylase that initiated transcript silencing, we depleted the known deadenylases in the gld-2(0) mutant background. Only the loss of CCF-1 suppressed the short-tailed phenotype of GLD-2 targets suggesting that in addition to its general role in RNA turnover, this is the major deadenylase for regulatory silencing of maternal mRNA. Analysis of poly(A)-tail length-change in the embryo lacking specific RNA-binding proteins revealed new candidates for asymmetric expression in the first embryonic divisions. CONCLUSION The concerted action of RNA binding proteins exquisitely regulates GLD-2 activity in space and time. We present our data as interactive web resources for a model where GLD-2 mediated cytoplasmic polyadenylation regulates target mRNA at each stage of worm germline and early embryonic development.

Related Papers

• → Global 3′ UTR shortening has a limited effect on protein abundance in proliferating T cells(2014)205 cited
• → Alternative polyadenylation in the regulation and dysregulation of gene expression(2017)86 cited
• → Post-transcriptional 3´-UTR cleavage of mRNA transcripts generates thousands of stable uncapped autonomous RNA fragments(2017)62 cited
• → Guinea Pig p53 mRNA: Identification of New Elements in Coding and Untranslated Regions and Their Functional and Evolutionary Implications(1999)21 cited
• Gambaran Pengetahuan, Sikap dan Perilaku Ibu hamil Terhadap Cakupan Kunjungan Pertama (K1) Di Puskesmas Lemah Wungkuk Kota Cirebon Tahun 2007(2000)