Unbiased identification of trans regulators of ADAR and A-to-I RNA editing
Citations Over Time
Abstract
Abstract Adenosine-to-Inosine RNA editing is catalyzed by ADAR enzymes that deaminate adenosine to inosine. While many RNA editing sites are known, few trans regulators have been identified. We perform BioID followed by mass-spectrometry to identify trans regulators of ADAR1 and ADAR2 in HeLa and M17 neuroblastoma cells. We identify known and novel ADAR-interacting proteins. Using ENCODE data we validate and characterize a subset of the novel interactors as global or site-specific RNA editing regulators. Our set of novel trans regulators includes all four members of the DZF-domain-containing family of proteins: ILF3, ILF2, STRBP, and ZFR. We show that these proteins interact with each ADAR and modulate RNA editing levels. We find ILF3 is a global negative regulator of editing. This work demonstrates the broad roles RNA binding proteins play in regulating editing levels and establishes DZF-domain containing proteins as a group of highly influential RNA editing regulators.
Related Papers
- → A comparative analysis of ADAR mutant mice reveals site-specific regulation of RNA editing(2020)58 cited
- → Controlling the Editor: The Many Roles of RNA-Binding Proteins in Regulating A-to-I RNA Editing(2016)13 cited
- → A comparative analysis among ADAR mutant mice reveals site-specific regulation of RNA editing(2019)1 cited
- The subcellular localization of ADAR determines A-to-I editing levels in developing neurons(2011)