Functional Annotation of Human Long Non-Coding RNAs via Molecular Phenotyping
Citations Over Time
Abstract
Abstract Long non-coding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes and yet, their functions remain largely unknown. We systematically knockdown 285 lncRNAs expression in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNA exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest to-date lncRNA knockdown dataset with molecular phenotyping (over 1,000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.
Related Papers
- → Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells(2016)60 cited
- → Long non-coding RNA FEZF1-AS1 promotes cell invasion and epithelial-mesenchymal transition through JAK2/STAT3 signaling pathway in human hepatocellular carcinoma(2018)37 cited
- Long non-coding RNA UCA1 promotes retinoblastoma progression by modulating the miR-124/c-myc axis.(2022)
- Knockdown of long noncoding RNA DLX6-AS1 inhibits cell proliferation and invasion of cervical cancer cells by downregulating FUS(2020)
- → Knockdown of long noncoding RNA 01124 inhibits the malignant behaviors of colon cancer cells by regulating miR-654-5p/HAX-1(2022)1 cited