Use of the BioGRID Database for Analysis of Yeast Protein and Genetic Interactions
Cold Spring Harbor Protocols2016Vol. 2016(1), pp. pdb.prot088880–pdb.prot088880
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Rose Oughtred, Andrew Chatr‐aryamontri, Bobby‐Joe Breitkreutz, Christie Chang, Jennifer Rust, Chandra L. Theesfeld, Sven Heinicke, Ashton Breitkreutz, Daici Chen, Jodi Hirschman, Nadine K. Kolas, Michael Livstone, Julie Nixon, Lara O’Donnell, Lindsay Ramage, Andrew Winter, Teresa Reguly, Adnane Sellam, Chris Stark, Lorrie Boucher, Kara Dolinski, Mike Tyers
Abstract
The BioGRID database is an extensive repository of curated genetic and protein interactions for the budding yeast Saccharomyces cerevisiae, the fission yeast Schizosaccharomyces pombe, and the yeast Candida albicans SC5314, as well as for several other model organisms and humans. This protocol describes how to use the BioGRID website to query genetic or protein interactions for any gene of interest, how to visualize the associated interactions using an embedded interactive network viewer, and how to download data files for either selected interactions or the entire BioGRID interaction data set.
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