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Staining Membrane-Bound Proteins with Coomassie Blue R250
Cold Spring Harbor Protocols2006Vol. 2006(5), pp. pdb.prot4544–pdb.prot4544
Abstract
INTRODUCTIONCoomassie Blue R250 permanently stains membrane-bound proteins and is compatible with PVDF and nitrocellulose membranes, but it is incompatible with nylon membranes. This technique is relatively insensitive, with a detection limit of ~1.5 μg of protein. One drawback of Coomassie Blue staining is that it produces a high background that can make interpretation of results difficult.
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