Sex Chromatin Staining in Amnion Cells
Cold Spring Harbor Protocols2008Vol. 2008(11), pp. pdb.prot5079–pdb.prot5079
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Abstract
INTRODUCTIONThe procedure described here provides a quick and reliable method for determining the sex of mouse embryos that are 95% concordance with genotyping data based on PCR for Y-chromosome sequences in extracted tail DNA. This protocol has the advantage of speed and efficiency: When assembling cultures with live tissue, samples can be sexed in 30 min.
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