Culturing the Moss Physcomitrella patens: Figure 1.
Cold Spring Harbor Protocols2009Vol. 2009(2), pp. pdb.prot5136–pdb.prot5136
Citations Over Time
David J. Cove, Pierre‐François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, Ralph S. Quatrano
Abstract
INTRODUCTION This article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by self-fertilization and sexual crossing.
Related Papers
- → Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens(2013)47 cited
- → Phylogenetic analyses of morphological evolution in the gametophyte and sporophyte generations of the moss order Hookeriales (Bryopsida)(2012)26 cited
- → Regeneration of Plants from Protoplasts of Mosses (Funaria hygrometrica and Physcomitrella patens)(1996)2 cited
- → The sporophyte-gametophyte junction in Hymenophyton and Symphyogyna (Metzgeriidae, Hepaticae): Structure and phylogenetic implications(1996)5 cited
- → Faculty Opinions recommendation of An ancient mechanism controls the development of cells with a rooting function in land plants.(2007)