Electroporation of Transgenic DNAs in the Sea Squirt Ciona
Cold Spring Harbor Protocols2009Vol. 2009(12), pp. pdb.prot5345–pdb.prot5345
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Abstract
INTRODUCTION The electroporation method described here is probably the mainstay of sea squirt (Ciona) research, because the utility of transgene expression in staged embryo populations enables a wide array of biological questions to be addressed. It allows rapid identification and characterization of cis -regulatory DNA, such as tissue-specific enhancers. Electroporation of plasmids expressing fluorescent reporter genes permits live imaging and lineage tracing. Finally, structure-function relationships can be examined by expressing dominant-negative or constitutively active forms of specific proteins using appropriate cell-specific enhancers.
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