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Trichloroacetic Acid (TCA) Precipitation of Proteins
Cold Spring Harbor Protocols2011Vol. 2011(8), pp. pdb.prot5651–pdb.prot5651
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Abstract
INTRODUCTION Cell or tissue extracts of proteins often contain interfering substances that must be removed. A number of methods are available for concentrating dilute protein solutions and simultaneously removing contaminating substances. Trichloroacetic acid (TCA)/acetone precipitation, as described here, is often used for this purpose.
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