The ArabidopsisSomatic Embryogenesis Receptor Kinase 1Gene Is Expressed in Developing Ovules and Embryos and Enhances Embryogenic Competence in Culture

Citations Over TimeTop 1% of 2001 papers

Abstract

We report here the isolation of the Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1 (AtSERK1) gene and we demonstrate its role during establishment of somatic embryogenesis in culture. The AtSERK1 gene is highly expressed during embryogenic cell formation in culture and during early embryogenesis. The AtSERK1 gene is first expressed in planta during megasporogenesis in the nucellus [corrected] of developing ovules, in the functional megaspore, and in all cells of the embryo sac up to fertilization. After fertilization, AtSERK1 expression is seen in all cells of the developing embryo until the heart stage. After this stage, AtSERK1 expression is no longer detectable in the embryo or in any part of the developing seed. Low expression is detected in adult vascular tissue. Ectopic expression of the full-length AtSERK1 cDNA under the control of the cauliflower mosaic virus 35S promoter did not result in any altered plant phenotype. However, seedlings that overexpressed the AtSERK1 mRNA exhibited a 3- to 4-fold increase in efficiency for initiation of somatic embryogenesis. Thus, an increased AtSERK1 level is sufficient to confer embryogenic competence in culture.

Related Papers

• → Embryo production through somatic embryogenesis can be used to study cell differentiation in plants(2006)262 cited
• → Characterization of a ZmSERK gene and its relationship to somatic embryogenesis in a maize culture(2010)70 cited
• → CORONA, PHABULOSA and PHAVOLUTA collaborate with BELL 1 to confine WUSCHEL expression to the nucellus in Arabidopsis ovules(2015)37 cited
• Changes of Embryo Protein Components During Early Somatic Embryogenesis of Litchi (Litchi chinensis Sonn.)(2012)
• Recent Advances in Conifer Somatic Embryogenesis(2004)