Resistance to Low Temperature Photoinhibition Is Not Associated with Isolated Thylakoid Membranes of Winter Rye
Citations Over Time
Abstract
In vivo measurements of chlorophyll a fluorescence indicate that cold-hardened winter rye (Secale cereale L. cv Musketeer) develops a resistance to low temperature-induced photoinhibition compared with nonhardened rye. After 7.2 hours at 5 degrees C and 1550 micromoles per square meter per second, the ratio of variable fluorescence/maximum fluorescence was depressed by only 23% in cold-hardened rye compared with 46% in nonhardened rye. We have tested the hypothesis that the principal site of this resistance to photoinhibition resides at the level of rye thylakoid membranes. Thylakoids were isolated from cold-hardened and nonhardened rye and exposed to high irradiance (1000-2600 micromoles per square meter per second) at either 5 or 20 degrees C. The photoinhibitory response measured by room temperature fluorescence induction, photosystem II electron transport, photoacoustic spectroscopy, or [(14)C]atrazine binding indicates that the differential resistance to low temperature-induced photoinhibition in vivo is not observed in isolated thylakoids. Similar results were obtained whether isolated rye thylakoids were photoinhibited or thylakoids were isolated from rye leaves preexposed to a photoinhibitory treatment. Thus, we conclude that increased resistance to low temperature-induced photoinhibition is not a property of thylakoid membranes but is associated with a higher level of cellular organization.
Related Papers
- → Application of low temperatures during photoinhibition allows characterization of individual steps in photodamage and the repair of photosystem II(2007)85 cited
- → Phase Transition of Thylakoid Membranes Modulates Photoinhibition in the Cyanobacterium Anabaena siamensis(2003)8 cited
- → Mechanism of copper‐enhanced photoinhibition in thylakoid membranes(2001)54 cited
- → Effects of bicarbonate and oxygen concentration on photoinhibition of thylakoid membranes(1992)14 cited
- → Photosynthetic Activity and Peroxidation of Thylakoid Lipids during Photoinhibition and High Temperature Treatment of Isolated Wheat Chloroplasts(1993)13 cited