Impact of adding an IgG4 conjugate to routine direct immunofluorescence testing for subepithelial and intraepithelial autoimmune blistering disorders
Journal of Cutaneous Pathology2021Vol. 49(4), pp. 358–362
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Julia S. Lehman, Emma F. Johnson, Michael Camilleri, Lawrence E. Gibson, Nneka I. Comfere, Amer N. Kalaaji, Margot S. Peters, Derek J. Cervenka, Joseph M. Doppler, Colleen R. Lange, C. Miller, Carilyn N. Wieland
Abstract
IgG4 allowed the classification of over 40% of DIF cases that were otherwise equivocal by IgG and C3. IgG4 staining showed lower levels of non-specific background staining than IgG or C3. IgG4 appears to contribute most value in cases with cell-surface deposition or with equivocal linear IgG deposition and negative C3 results.
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