Development of 3D imaging technique of reconstructed human epidermis with immortalized human epidermal cell line
Citations Over Time
Abstract
The epidermis, the outermost layer of the skin, retains moisture and functions as a physical barrier against the external environment. Epidermal cells are continuously replaced by turnover, and thus to understand in detail the dynamic cellular events in the epidermis, techniques to observe live tissues in 3D are required. Here, we established a live 3D imaging technique for epidermis models. We first obtained immortalized human epidermal cell lines which have a normal differentiation capacity and fluorescence-labelled cytoplasm or nuclei. The reconstituted 3D epidermis was prepared with these lines. Using this culture system, we were able to observe the structure of the reconstituted epidermis live in 3D, which was similar to an in vivo epidermis, and evaluate the effect of a skin irritant. This technique may be useful for dermatological science and drug development.
Related Papers
- → Comparison of gene expression profiles in human keratinocyte mono‐layer cultures, reconstituted epidermis and normal human skin; transcriptional effects of retinoid treatments in reconstituted human epidermis(2002)91 cited
- → Preparation of Human Epidermal Keratinocyte Cultures(2003)7 cited
- → Stereotyped Distribution of Proliferating Keratinocytes in Disorders Affecting the Epidermis(1989)3 cited
- → Keratinocyte protein expression in rapidly regenerating epidermis following laser‐induced thermal injury(1989)1 cited
- → 890 Development of pigmented reconstructed human epidermis model containing human melanoblasts from keratinocyte culture(2019)