Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice
Journal of Applied Microbiology2016Vol. 120(5), pp. 1357–1367
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Zhouqi Cui, Mohammad Reza Ojaghian, Tao Zheng, Kaleem U. Kakar, Jiamei Zeng, Wenjun Zhao, Y. P. Duan, C. M. Vera Cruz, Bin Li, Bo Zhu, G. L. Xie
Abstract
This study is the first report of a method allowing simultaneous detection of six major rice pathogens. The ability to use crude extracts from plants without bacterial isolation or DNA extraction enhances the value of this mPCR technology for rapid detection and aetiological/epidemiological studies.
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