Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets
Citations Over TimeTop 19% of 2018 papers
Abstract
Background Extracellular vesicles (EVs) from human saliva expose coagulant tissue factor (TF). Whether such TF-exposing EVs contribute to hemostasis, however, is unknown. Recently, in a mice model, tumor cell-derived EVs were shown to deliver coagulant TF to activated platelets at a site of vascular injury via interaction between P-selectin glycoprotein ligand-1 (PSGL-1) and P-selectin. Objectives We hypothesized that salivary EVs may deliver coagulant TF to activated platelets via interaction with P-selectin. Methods We investigated the presence of two ligands of P-selectin on salivary EVs, PSGL-1 and CD24. Results Salivary EVs expose CD24 but PSGL-1 was not detected. Immune depletion of CD24-exposing EVs completely abolished the TF-dependent coagulant activity of cell-free saliva, showing that coagulant TF and CD24 co-localize on salivary EVs. In a whole blood perfusion model, salivary EVs accumulated at the surface of activated platelets and promoted fibrin generation, which was abolished by an inhibitory antibody against human CD24. Conclusions A subset of EVs in human saliva expose coagulant TF and CD24, a ligand of P-selectin, suggesting that such EVs may facilitate hemostasis at a site of skin injury where the wound is licked in a reflex action.
Related Papers
- → Mouse models of hemostasis(2020)55 cited
- → Hemostasis and Platelet Dysfunction in Asphyxiated Neonates(2011)51 cited
- Hemostasis Effect of Disposable Packing Hemostasis on Miniature Pigs(2010)
- → Sequential Formation Of Des-A Fibrin And Des-Ab Fibrin And Binding Of Fibrinogen To Des-Ab Fibrin(1981)
- → High Fibrinogen (Fg) Content in Plasma Clots Harvested at Arrested, Incipient Coagulation(1979)