Rapid and accurate identification of Xanthomonas citri subspecies citri by fluorescence in situ hybridization
Letters in Applied Microbiology2016Vol. 63(5), pp. 315–321
Citations Over TimeTop 10% of 2016 papers
Abstract
Xanthomonas citri subsp. citri (Xcc) is an aggressive and hardy pathogen of citrus plants worldwide. Outbreaks are difficult and costly to contain and the establishment of citrus canker results in restricted trade. In order to extend the existing toolkit for identification of Xcc we developed a novel diagnostic approach based on fluorescence in situ hybridization. Our approach is of comparable specificity and sensitivity to existing methods but can be performed directly on infected tissue making it significantly faster than existing PCRs, and requiring fewer laboratory resources.
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