Why the COI barcode should be the community DNA metabarcode for the metazoa
Citations Over TimeTop 1% of 2018 papers
Abstract
Metabarcoding of complex metazoan communities is increasingly being used to measure biodiversity in terrestrial, freshwater and marine ecosystems, revolutionizing our ability to observe patterns and infer processes regarding the origin and conservation of biodiversity. A fundamentally important question is which genetic marker to amplify, and although the mitochondrial cytochrome oxidase subunit I (COI) gene is one of the more widely used markers in metabarcoding for the Metazoa, doubts have recently been raised about its suitability. We argue that (a) the extensive coverage of reference sequence databases for COI; (b) the variation it presents; (c) the comparative advantages for denoising protein-coding genes; and (d) recent advances in DNA sequencing protocols argue in favour of standardizing for the use of COI for metazoan community samples. We also highlight where research efforts should focus to maximize the utility of metabarcoding.
Related Papers
- → How much metagenomic sequencing is enough to achieve a given goal?(2013)104 cited
- → Application of metagenomic next-generation sequencing and targeted metagenomic next-generation sequencing in diagnosing pulmonary infections in immunocompetent and immunocompromised patients(2024)28 cited
- → Red Sea fishes in the Mediterranean Sea: a preliminary investigation of a biological invasion using DNA barcoding(2015)59 cited
- → Cytochrome c oxidase subunit I barcoding of the green bee-eater (Merops orientalis)(2011)13 cited
- DNA Barcoding on the Identification of 8 Species of Raptors(2009)