The desert plant Phoenix dactylifera closes stomata via nitrate‐regulated SLAC1 anion channel
Citations Over TimeTop 10% of 2017 papers
Abstract
Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss? Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P. dactylifera. To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid (ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The PhoenixSLAC1-type anion channel is regulated by ABA kinase PdOST1. Energy-dispersive X-ray analysis (EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl- medium, PdOST1 did not activate the desert plant anion channel PdSLAC1 per se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated PdSLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure. Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium.
Related Papers
- → Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport(2021)34 cited
- → The site of action of abscisic acid at the guard cell plasmalemma of Valerianella locusta(1983)141 cited
- 보문 (報文) : 둥근바위솔 ( Orostachys malacophyllus ) 잎의 표피구조와 기공발생에 미치는 Abscisic Acid 의 영향(1987)
- → Guard Cell Response(2000)
- → Guard Cell Response(2000)