PdGNC confers drought tolerance by mediating stomatal closure resulting from NO and H2O2 production via the direct regulation of PdHXK1 expression in Populus
Citations Over TimeTop 1% of 2021 papers
Abstract
Summary Drought is one of the primary abiotic stresses, seriously implicating plant growth and productivity. Stomata play a crucial role in regulating drought tolerance. However, the molecular mechanism on stomatal movement‐mediated drought tolerance remains unclear. Using genetic, molecular and biochemical techniques, we identified that the PdGNC directly activating the promoter of PdHXK1 by binding the GATC element, a hexokinase (HXK) synthesis key gene. Here, PdGNC , a member of the GATA transcription factor family, was greatly induced by abscisic acid and dehydration. Overexpressing PdGNC in poplar ( Populus clone 717) resulted in reduced stomatal aperture with greater water‐use efficiency and increased water deficit tolerance. By contrast, CRISPR/Cas9‐mediated poplar mutant gnc exhibited increased stomatal aperture and water loss with reducing drought resistance. PdGNC activates PdHXK1 (a hexokinase synthesis key gene), resulting in a remarkable increase in hexokinase activity in poplars subjected to water deficit. Furthermore, hexokinase promoted nitric oxide (NO) and hydrogen peroxide (H 2 O 2 ) production in guard cells, which ultimately reduced stomatal aperture and increased drought resistance. Together, PdGNC confers drought stress tolerance by reducing stomatal aperture caused by NO and H 2 O 2 production via the direct regulation of PdHXK1 expression in poplars.
Related Papers
- A Review of Mine Closure Planning and Practice in Canada and Australia(2015)
- → Bi-closure systems and bi-closure operators on generalized residuated lattices(2019)2 cited
- Advances in the studies of function genes for drought tolerance(2004)
- Guidance manual on hazardous-waste land treatment closure/post-closure, 40 CFR Part 265. Final report(1987)
- → One-step closure, weak one-step closure and meet continuity(2022)