A novel aphid resistance locus in cowpea identified by combining SSR and SNP markers

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Abstract

Abstract The utility of combining simple sequence repeat ( SSR ) and single nucleotide polymorphism ( SNP ) marker genotyping was determined for genetically mapping a novel aphid ( Aphis craccivora ) resistance locus in cowpea breeding line SARC 1‐57‐2 and for introgressing the resistance into elite cultivars by marker‐assisted backcrossing ( MABC ). The locus was tagged with codominant SSR marker CP 171F/172R with a recombination fraction of 5.91% in an F 2 population from ‘Apagbaala’ x SARC 1‐57‐2. A SNP ‐genotyped biparental recombinant inbred line population was genotyped for CP 171F/172R, which was mapped to position 11.5 cM on linkage group ( LG ) 10 (physical position 30.514 Mb on chromosome Vu10). Using CP 171F/172R for foreground selection and a KASP ‐ SNP ‐based marker panel for background selection in MABC , the resistance from SARC 1‐57‐2 was introduced into elite susceptible cultivar ‘Zaayura’. Five BC 4 F 3 lines of improved ‘Zaayura’ that were isogenic except for the resistance locus region had phenotypes similar to SARC 1‐57‐2. This study identified a novel aphid resistance locus and demonstrated the effectiveness of integrating SSR and SNP markers for trait mapping and marker‐assisted breeding.

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