Isoprenylation modification is required for HIPP1‐mediated powdery mildew resistance in wheat
Citations Over TimeTop 10% of 2022 papers
Abstract
Powdery mildew (Pm), caused by Blumeria graminis f.sp. tritici (Bgt), is one of the most important wheat diseases. Heavy-metal-associated isoprenylated plant protein (HIPP1) has been proved playing important roles in response to biotic and a biotic stress. In present study, we proved HIPP1-V from Haynalidia villosa is a positive regulator in Pm resistance. HIPP1-V was rapidly induced by Bgt. Transiently or stably heterologous overexpressing HIPP1-V in wheat suppressed the haustorium formation and enhanced Pm resistance. HIPP1-V isoprenylation was critical for plasma membrane (PM) localization, interaction with E3-ligase CMPG1-V and function in Pm resistance. Bgt infection recruited the isoprenylated HIPP1-V and CMPG1s on PM; blocking the HIPP1 isoprenylation reduced such recruitment and compromised the resistance of OE-CMPG1-V and OE-HIPP1-V. Overexpressing HIPP1-VC148G could not enhance Pm resistance. These indicated the Pm resistance was dependent on HIPP1-V's isoprenylation. DGEs related to the ROS and SA pathways were remarkably enriched in OE-HIPP1-V, revealing their involvement in Pm resistance. Our results provide evidence on the important role of protein isoprenylation in plant defense.
Related Papers
- → Sensitivity of Wheat Powdery Mildew (Blumeria graminis f.sp. tritici) towards Metrafenone(2010)26 cited
- → Mlo Resistance to Powdery Mildew (Blumeria graminis f. sp. hordei) in Barley Landraces Collected in Yemen(2021)11 cited
- → Virulence structure of the Blumeria graminis DC.f. sp. avenae populations occurring in Poland across 2010–2013(2017)22 cited
- → Identification of a novel recessive gene for resistance to powdery mildew (Blumeria graminis f. sp. hordei) in barley (Hordeum vulgare)(2020)16 cited
- → Selections from barley landrace collected in Libya as new sources of effective resistance to powdery mildew (Blumeria graminis f.sp. hordei)(2002)6 cited