Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme , one causal agent of sudden death syndrome

Citations Over TimeTop 10% of 2018 papers

Abstract

Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome ( SDS ) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS . Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme , was observed in the presence of arbuscular mycorrhizal fungi ( AMF ), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme , grown under strict in vitro culture experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF ‐colonized (+ AMF +Fv) and noncolonized (− AMF +Fv) plants, respectively. Major transcriptional changes corresponded to defence response‐related genes belonging to secondary metabolism, stress and signalling categories. The + AMF +Fv treatment showed the largest number of up‐regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the + AMF +Fv treatment, such as those encoding a thaumatin‐like protein ( TLP ) and a pleiotropic drug resistance ( PDR ) protein. Moreover, + AMF +Fv showed a significantly greater number of down‐regulated genes related to cell wall modification and peroxidases than the – AMF +Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme .

Related Papers

• → OsWRKY53, a versatile switch in regulating herbivore-induced defense responses in rice(2016)76 cited
• → Defense-Related Proteins in Sugarcane and Their Role in Disease Resistance: Molecular Advancements and Beyond(2022)4 cited
• → Characteristic Expression Analysis of Five WRKY Transcriptional Factors in Rice Leaf Growth and Disease Resistance Reaction(2013)2 cited
• → Transcription factor ScWRKY4 in sugarcane negatively regulates the resistance to pathogen infection through the JA signaling pathway(2023)