Metabolic differentiation and intercellular nurturing underpin bacterial endospore formation
Citations Over TimeTop 16% of 2021 papers
Abstract
Despite intensive research, the role of metabolism in bacterial sporulation remains poorly understood. Here, we demonstrate that Bacillus subtilis sporulation entails a marked metabolic differentiation of the two cells comprising the sporangium: the forespore, which becomes the dormant spore, and the mother cell, which dies as sporulation completes. Our data provide evidence that metabolic precursor biosynthesis becomes restricted to the mother cell and that the forespore becomes reliant on mother cell-derived metabolites for protein synthesis. We further show that arginine is trafficked between the two cells and that proposed proteinaceous channels mediate small-molecule intercellular transport. Thus, sporulation entails the profound metabolic reprogramming of the forespore, which is depleted of key metabolic enzymes and must import metabolites from the mother cell. Together, our results provide a bacterial example analogous to progeny nurturing.
Related Papers
- → Bacillus subtilis Endospores at High Purity and Recovery Yields: Optimization of Growth Conditions and Purification Method(2012)65 cited
- → Direct reprogramming 101(2010)20 cited
- → ВПЛИВ BACILLUS SUBTILIS НА ПОРОСЯТ НА ВІДЛУЧЕННІ(2022)4 cited
- → СВОЙСТВА ШТАММОВ BACILLUS SUBTILIS КАК ПРОДУЦЕНТОВ АМИЛАЗ ПРИ ПРОИЗВОДСТВЕ САХАРОСОДЕРЖАЩЕЙ КОРМОВОЙ ДОБАВКИ(2020)1 cited
- 산란계에 Bacillus subtilis의 급여가 계란 품질, 혈액 성상 및 분내 암모니아태 질소 함량에 미치는 영향(2005)