The Transcriptional Landscape of the Yeast Genome Defined by RNA Sequencing
Citations Over TimeTop 1% of 2008 papers
Abstract
The identification of untranslated regions, introns, and coding regions within an organism remains challenging. We developed a quantitative sequencing-based method called RNA-Seq for mapping transcribed regions, in which complementary DNA fragments are subjected to high-throughput sequencing and mapped to the genome. We applied RNA-Seq to generate a high-resolution transcriptome map of the yeast genome and demonstrated that most (74.5%) of the nonrepetitive sequence of the yeast genome is transcribed. We confirmed many known and predicted introns and demonstrated that others are not actively used. Alternative initiation codons and upstream open reading frames also were identified for many yeast genes. We also found unexpected 3'-end heterogeneity and the presence of many overlapping genes. These results indicate that the yeast transcriptome is more complex than previously appreciated.
Related Papers
- → Intron-dependent transient expression of the maize GapA1 gene(1995)48 cited
- → Stimulation of gene expression by introns: conversion of an inhibitory intron to a stimulatory intron by alteration of the splice donor sequence(1993)38 cited
- → Correlation between splicing sites within an intron and their sequence complementarity with U1 RNA(1980)92 cited
- → The inconsistent distribution of introns in the T-even phages indicates recent genetic exchanges(1989)39 cited
- Effects of splice sites on the intron retention in histamine H3 receptors from rats and mice(2009)