Determination of Macromolecular Structures from Anomalous Diffraction of Synchrotron Radiation
Citations Over TimeTop 1% of 1991 papers
Abstract
Resonance between beams of x-ray waves and electronic transitions from bound atomic orbitals leads to a phenomenon known as anomalous scattering. This effect can be exploited in x-ray crystallographic studies on biological macromolecules by making diffraction measurements at selected wavelengths associated with a particular resonant transition. In this manner the problem of determining the three-dimensional structure of thousands of atoms is reduced to that of initially solving for a few anomalous scattering centers that can then be used as a reference for developing the entire structure. This method of multiwavelength anomalous diffraction has now been applied in a number of structure determinations. Optimal experiments require appropriate synchrotron instrumentation, careful experimental design, and sophisticated analytical procedures. There are rich opportunities for future applications.
Related Papers
- Anomalous X-ray scattering for materials characterization : atomic-scale structure determination(2002)
- → Experimental investigation of far-field diffraction by means of normally and non-normally illuminated elliptical apertures of wavelength dimension(1994)7 cited
- → Diffraction by Apertures of Wavelength Dimensions(1965)7 cited
- → X-Ray Anomalous Scattering Factors — Measurements and Applications(1981)5 cited
- → Frontiers in Crystallography with Synchrotron Radiation. X-ray Studies near Absorption Edges of Elements. Atom-Selective Experiments. Detection of Structural Fluctuations in Materials with the Use of X-Ray Anomalous Scattering Phenomena.(1997)