A three-dimensional movie of structural changes in bacteriorhodopsin
Science2016Vol. 354(6319), pp. 1552–1557
Citations Over TimeTop 1% of 2016 papers
Eriko Nango, Antoine Royant, Minoru Kubo, Takanori Nakane, Cecilia Wickstrand, Tetsunari Kimura, Tomoyuki Tanaka, Kensuke Tono, Changyong Song, Rie Tanaka, Toshi Arima, A. Yamashita, Jun Kobayashi, Toshiaki Hosaka, Eiichi Mizohata, Przemysław Nogły, Michihiro Sugahara, Daewoong Nam, Takashi Nomura, Tatsuro Shimamura, Dohyun Im, Takaaki Fujiwara, Yasuaki Yamanaka, Byeonghyun Jeon, Tomohiro Nishizawa, Kazumasa Oda, Masahiro Fukuda, Rebecka Andersson, Petra Båth, Robert Dods, Jan Davidsson, Shigeru Matsuoka, Satoshi Kawatake, Michio Murata, Osamu Nureki, Shigeki Owada, Takashi Kameshima, Takaki Hatsui, Yasumasa Joti, Gebhard F. X. Schertler, Makina Yabashi, Ana‐Nicoleta Bondar, Jörg Standfuss, Richard Neutze, So Iwata
Abstract
Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.
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