Fine mapping of an immunoglobulin gene activator.

Citations Over TimeTop 1% of 1984 papers

Abstract

It has recently been shown that the promoter of a kappa immunoglobulin gene is activated for transcription by a downstream sequence element. Here we mapped the activating element to a resolution of about 20 base pairs by constructing a series of deletions in the cloned kappa gene. After transfection of each deleted gene into myeloma cells, a transient expression assay was used to measure the level of transcription from the kappa promoter. We found that the activating element extends through about 200 base pairs and encompasses a region of sequence that is conserved between mouse and human genes. As successively deeper deletions were made into the conserved region from either the 5' or 3' side, the activating ability was lost gradually rather than abruptly. Although several short segments in this region are homologous to sequences in viral enhancers, they did not seem to play a dominant role in the activating effect. We also found that the activating element remained functional when reversed in orientation or when moved upstream of the kappa gene.

Related Papers

• → The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat(1985)820 cited
• → De Novo Genesis of Enhancers in Vertebrates(2011)66 cited
• → The 5′ Region of the Human Papillomavirus Type 31 Upstream Regulatory Region Acts as an Enhancer Which Augments Viral Early Expression Through the Action of YY1(1997)40 cited
• → Repression precedes independent evolutionary gains of a highly specific gene expression pattern(2021)5 cited
• → Evolution of transposable element-derived enhancer activity(2022)1 cited