Developmental potentialities of leaf primordia of Osmunda cinnamomea. II. Further studies on the influence of determined leaf primordia on undetermined leaf primordia

Citations Over TimeTop 10% of 1969 papers

Abstract

In the fern Osmunda cinnamomea, leaf primordia may be excised and grown in sterile culture before or after their irreversible determination as leaves. It has been demonstrated that P 3 primordia (third most recently formed primordia) are not irreversibly determined as leaves at the time of excision for they exhibit a tendency to develop as whole shoots (approximately 67.0% of the time) whether grown singly, or as pairs grown in physiological contact. Results from the present investigation support the hypothesis that a morphogenetic factor(s) is found in older primorida which is transmitted to younger primordia to influence the latter to develop as leaves rather than shoots, for P 3 's grown in physiological contact (as pairs) with P 10 , P 12 , or P 13 primordia are expressed as leaves at a level approximately twofold or greater than P 3 's grown singly (controls). Results from the present investigation do not support the hypothesis that increased bulk of tissue, and therefore increased capacity for nutrient productivity by older and larger primordia, is responsible for imposition of leafness on undetermined P 3 primordia, for P 14 primordia grown in physiological contact with P 3 primordia (as pairs) are observed to increase the percentage of P 3 's expressing leafness at a level only slightly greater than P 3 's grown singly, and slightly less than when P 3 's are grown in physiological contact with other P 3 's as pairs. The slight enhancement in leafness exhibited by undetermined P 3 's grown as pairs with other P 3 's, or with P 14 's has been shown to be non-significant at the 5% level (P < 0.05) by the standard t test and Kramer's (1956) modification of Duncan's (1955) new multiple range test.

Related Papers

• → Photoinduction of Flower Identity in Vegetatively Biased Primordia(1998)52 cited
• → DEVELOPMENTAL POTENTIALITIES OF LEAF PRIMORDIA OF OSMUNDA CINNAMOMEA: THE INFLUENCE OF DETERMINED LEAF PRIMORDIA ON UNDETERMINED LEAF PRIMORDIA(1967)14 cited
• → Mass Propagation of Multiple Shoots Using a Large Bioreactor.(1994)1 cited
• Studies on Dynamic Models and Characteristics of Apex Primordium Differentiation in Wheat(2000)