Anther, ovule, seed, and nucellar embryo development inCitrus sinensiscv. Valencia

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Abstract

'Valencia' orange, a commercially important cultivar of Citrus, forms polyembryonic seeds by an apomictic process called nucellar embryony in which many embryos initiate directly from nucellar cells surrounding the sexual embryo sac. We observed anther, ovule, seed, and fruit development in relation to nucellar embryo development in seeds and unfertilized ovules of 'Valencia'. Pollination and fertilization are required to set fruit in 'Valencia', and low seed set was found to be related to defects in both male and female gametogenesis. Nucellar embryo initial cells were evident histologically in ovules of flowers just prior to anthesis. However, in vitro culture of ovules from flowers at different prepollination stages showed that embryos could develop from ovules cultured as early as the binucleate stage of megagametogenesis in which nucellar initial cells were absent histologically. During fruit development, the timing and sequence of the early events of nucellar embryo formation were synchronous in seeds and unfertilized ovules, indicating a co-ordinated control of embryo development in spatially and developmentally distinct structures. In both developing seeds and unfertilized ovules, embryo initial cells first formed thick walls, which isolated them from surrounding maternal tissue. In later stages, the cell walls thinned in some initial cells and embryogenesis became asynchronous. Cleavage of embryogénie cells coincided with degenerative processes linked to embryo sac expansion in seeds and to a previously unreported, localized degeneration in the central portion of the nucellus in unfertilized ovules. Some initial cells never divided. Nucellar embryo development was restricted to the central portion of unfertilized ovules and to the micropylar region of seeds. Only fertilized ovules had the capacity to form mature polyembryonic seeds. In unfertilized ovules a specialized vascular structure formed linking developing embryos to the chalazal vasculature of the ovule. Embryo development arrested at the globular stage in unfertilized ovules and the integuments differentiated to form a seed coat. The timing of reproductive events described was linked to floral and fruit morphological characteristics to facilitate molecular characterization of nucellar embryogenesis and seed formation in this cultivar. Key words: Citrus, nucellar embryony, seed, ovule, apomixis.

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